Regulatory

Part:BBa_K4829004:Experience

Designed by: Modernatx   Group: iGEM23_IISc-Bengaluru   (2023-10-04)


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Applications of BBa_K4829004

This can be incorporated in mRNA designs as done in BBa K4829003. We have used this UTR in the same fashion. We saw expression of the required protein in a HeLa cell culture 6hrs post-transfection (with lipofectamine MessengerMax). (1:1 ratio of the mRNA and lipofectamine).

  • hela-blot.png
 Figure 1. Expression of the protein noted in the supernatant 6 hours post transfection ]]

User Reviews

UNIQ12909ed7b63cdb79-partinfo-00000000-QINU UNIQ12909ed7b63cdb79-partinfo-00000001-QINU